5 years ago

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria [Plant Biology]

Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria [Plant Biology]
Kevin Baudry, Benȷamin Broche, Etienne Delannoy, Geert De Jaeger, Richard Berthome, Andeol Falcon de Longevialle, Damien Guillaumot, Guillem Rigaill, Claire Lurin, Alexandra Avon, Mauricio Lopez–Obando, Mizuki Takenaka

RNA editing is converting hundreds of cytosines into uridines during organelle gene expression of land plants. The pentatricopeptide repeat (PPR) proteins are at the core of this posttranscriptional RNA modification. Even if a PPR protein defines the editing site, a DYW domain of the same or another PPR protein is believed to catalyze the deamination. To give insight into the organelle RNA editosome, we performed tandem affinity purification of the plastidial CHLOROPLAST BIOGENESIS 19 (CLB19) PPR editing factor. Two PPR proteins, dually targeted to mitochondria and chloroplasts, were identified as potential partners of CLB19. These two proteins, a P-type PPR and a member of a small PPR-DYW subfamily, were shown to interact in yeast. Insertional mutations resulted in embryo lethality that could be rescued by embryo-specific complementation. A transcriptome analysis of these complemented plants showed major editing defects in both organelles with a very high PPR type specificity, indicating that the two proteins are core members of E+-type PPR editosomes.

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