3 years ago

Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis

Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis
Eun-Kyung Lim, Hye-Young Son, Taejoon Kang, Miyeon Lee, Jeong Moon, Jinyoung Jeong, Hongki Kim, Bongsoo Kim, Min-Kyo Seo, Yuna Choi, Gayoung Eom, Yong-Min Huh, Ahreum Hwang, Donghyeong Kim
Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nanoparticles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL−1. Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing. A nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is developed for ultrasensitive and specific detection of telomerase activity. The optimized nanogap-rich Au NW can provide highly reproducible and strong SERS signals. Using nanogap-rich Au NW SERS sensors, telomerase activities of cancer cells and tumor tissues are successfully detected, suggesting the potential applicability of nanogap-rich Au NW SERS sensor in cancer diagnosis.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/adfm.201701832

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