Journal of the American Chemical Society
Journal of the American Chemical Society
3 years ago

Fast Electrochemical and Plasmonic Detection Reveals Multitime Scale Conformational Gating of Electron Transfer in Cytochrome c

Fast Electrochemical and Plasmonic Detection Reveals Multitime Scale Conformational Gating of Electron Transfer in Cytochrome c
Hong-Yuan Chen, Hui Wang, Xiaonan Shan, Yan Wang, Yixian Wang, Nongjian Tao, Yuheng Chen
Conformational fluctuations play a central role in the electron transfer reactions of molecules. Because the fluctuations can be extremely fast in kinetics and small in amplitude, a technique with fast temporal resolution and high conformational sensitivity is needed to follow the transient electron transfer processes. Here we report on an electrochemically controlled plasmonic detection technique capable of monitoring conformational changes in redox molecules with ns response time. Using the technique, we study the electron transfer reaction and the associated conformational gating of a redox protein (cytochrome c). The study reveals that the conformational gating takes place over a broad range of time scales, from microsecond to millisecond.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b00839

DOI: 10.1021/jacs.7b00839

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