Chemical Science
Chemical Science
5 years ago

Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis

Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis
Kiyohiko Igarashi, Takashi Hayashi, Masahiro Samejima, Takayuki Uchihashi, Akira Onoda, Hirofumi Harada, Hiroki Watanabe, Naoki Sunagawa
To visualize the dynamic domain motion of class-I CDH from Phanerochaete chrysosporium (PcCDH) during catalysis using high-speed atomic force microscopy, the apo-form of PcCDH was anchored to a heme-immobilized flat gold surface that can fix the orientation of the CYT domain.

Publisher URL: http://feeds.rsc.org/~r/rss/SC/~3/esvP9xiw-5g/C7SC01672G

DOI: 2017/SC/C7SC01672G

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