5 years ago

An Approach to the High-Throughput Fabrication of Glycopolymer Microarrays through Thiol–Ene Chemistry

An Approach to the High-Throughput Fabrication of Glycopolymer Microarrays through Thiol–Ene Chemistry
Jin Geng, Andrea Venturato, Matthew Owens, Kevin Neumann, Yichuan Zhang, Mark Bradley, Antonio Conde-González
The fabrication of microarrays consisting of well-defined glycopolymers is described. This was achieved by postfunctionalization of an immobilized poly(allyl glycidyl ether) using unprotected thiol-modified carbohydrates through thiol–ene conjugation chemistry. This enabled the fabrication of glycopolymer microarrays in which the density and composition of the carbohydrate moieties varied along each of the polymer chains displayed across the array. These glycopolymer microarrays were applied in the evaluation of multivalent ligand–protein interactions with the determination of surface dissociation constants (KD) with concanavalin A and Ricinus communis agglutinin I for surface immobilized mannose-, glucose-, and galactose-containing glycopolymers and validated in solution with ITC.

Publisher URL: http://dx.doi.org/10.1021/acs.macromol.7b00952

DOI: 10.1021/acs.macromol.7b00952

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