5 years ago

Impact of prior flavivirus immunity on Zika virus infection in rhesus macaques

Anna B. Mullins, Xiaoxu Lin, Jeffrey R. Currier, Michael Boyd, Michael K. McCracken, Peter Abbink, Gregory D. Gromowski, Michelle Liu, Kenneth H. Eckles, Richard G. Jarman, Stephen J. Thomas, Rafael De La Barrera, Heather L. Friberg, Mark Milazzo, David Jetton, Dan H. Barouch, J. Robert Putnak, Kayvon Modjarrad, Lindsey S. Garver, Bridget S. Lewis, Matthew C. Wise, Nelson L. Michael

by Michael K. McCracken, Gregory D. Gromowski, Heather L. Friberg, Xiaoxu Lin, Peter Abbink, Rafael De La Barrera, Kenneth H. Eckles, Lindsey S. Garver, Michael Boyd, David Jetton, Dan H. Barouch, Matthew C. Wise, Bridget S. Lewis, Jeffrey R. Currier, Kayvon Modjarrad, Mark Milazzo, Michelle Liu, Anna B. Mullins, J. Robert Putnak, Nelson L. Michael, Richard G. Jarman, Stephen J. Thomas

Studies have demonstrated cross-reactivity of anti-dengue virus (DENV) antibodies in human sera against Zika virus (ZIKV), promoting increased ZIKV infection in vitro. However, the correlation between in vitro and in vivo findings is not well characterized. Thus, we evaluated the impact of heterotypic flavivirus immunity on ZIKV titers in biofluids of rhesus macaques. Animals previously infected (≥420 days) with DENV2, DENV4, or yellow fever virus were compared to flavivirus-naïve animals following infection with a Brazilian ZIKV strain. Sera from DENV-immune macaques demonstrated cross-reactivity with ZIKV by antibody-binding and neutralization assays prior to ZIKV infection, and promoted increased ZIKV infection in cell culture assays. Despite these findings, no significant differences between flavivirus-naïve and immune animals were observed in viral titers, neutralizing antibody levels, or immune cell kinetics following ZIKV infection. These results indicate that prior infection with heterologous flaviviruses neither conferred protection nor increased observed ZIKV titers in this non-human primate ZIKV infection model.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.ppat.1006487

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.