5 years ago

GTP Hydrolysis by eIF5B in the Last Step of Translation Initiation Is Activated by a Rotation of the Small Ribosomal Subunit.

V., Ramakrishnan, I. S., Fernandez
Placement of an initiator aminoacyl-tRNA [(f)Met-tRNAi(f)Met] base paired with the AUG initiation codon of a messenger RNA (mRNA) is the first step of translation. The eukaryotic translation factor eIF5B or its bacerial homologue IF2 facilitate the correct positioning of initiator tRNA in the P site of the ribosome. We report the electron cryo-microscopy (cryoEM) structure of a stabilized intermediate state of a yeast 80S/tRNAiMet/eIF5B complex at 3.6A resolution. The structure shows how a universally conserved tyrosine couples the rotational state of the small ribosomal subunit with GTP hydrolysis.

Publisher URL: http://biorxiv.org/cgi/content/short/172825v1

DOI: 10.1101/172825

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