5 years ago

Efficient Dual siRNA and Drug Delivery Using Engineered Lipoproteoplexes

Efficient Dual siRNA and Drug Delivery Using Engineered Lipoproteoplexes
P. Douglas Renfrew, Lindsay K. Hill, Nikita Srivastava, Liming Yin, Priya Katyal, Che Fu Liu, Joseph A. Frezzo, Richard Bonneau, Jin Kim Montclare, Haresh T. More, Raymond Chen
An engineered supercharged coiled-coil protein (CSP) and the cationic transfection reagent Lipofectamine 2000 are combined to form a lipoproteoplex for the purpose of dual delivery of siRNA and doxorubicin. CSP, bearing an external positive charge and axial hydrophobic pore, demonstrates the ability to condense siRNA and encapsulate the small-molecule chemotherapeutic, doxorubicin. The lipoproteoplex demonstrates improved doxorubicin loading relative to Lipofectamine 2000. Furthermore, it induces effective transfection of GAPDH (60% knockdown) in MCF-7 breast cancer cells with efficiencies comparing favorably to Lipofectamine 2000. When the lipoproteoplex is loaded with doxorubicin, the improved doxorubicin loading (∼40 μg Dox/mg CSP) results in a substantial decrease in MCF-7 cell viability.

Publisher URL: http://dx.doi.org/10.1021/acs.biomac.7b00203

DOI: 10.1021/acs.biomac.7b00203

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