Monitoring Hydrogen Exchange During Protein Folding by Fast Pressure Jump NMR Spectroscopy

Journal of the American Chemical Society

5 years ago

Monitoring Hydrogen Exchange During Protein Folding by Fast Pressure Jump NMR Spectroscopy

Philip Anfinrud, T. Reid Alderson, Ad Bax, Cyril Charlier, Dennis A. Torchia

A method is introduced that permits direct observation of the rates at which backbone amide hydrogens become protected from solvent exchange after rapidly dropping the hydrostatic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions. The method is demonstrated for a pressure-sensitized ubiquitin variant that contains two Val to Ala mutations. Increased protection against hydrogen exchange with solvent is monitored as a function of time during the folding process. Results for 53 backbone amides show narrow clustering with protection occurring with a time constant of ca. 85 ms, but slower protection is observed around a reverse turn near the C-terminus of the protein. Remarkably, the native NMR spectrum returns with this slower time constant of ca. 150 ms, indicating that the almost fully folded protein retains molten globule characteristics with severe NMR line broadening until the final hydrogen bonds are formed. Prior to crossing the transition state barrier, hydrogen exchange protection factors are close to unity, but with slightly elevated values in the β₁–β₂ hairpin, previously shown to be already lowly populated in the urea-denatured state.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b06676

DOI: 10.1021/jacs.7b06676