Journal of the American Chemical Society
Journal of the American Chemical Society
5 years ago

Site-Selective Sensing of Histone Methylation Enzyme Activity via an Arrayed Supramolecular Tandem Assay

Site-Selective Sensing of Histone Methylation Enzyme Activity via an Arrayed Supramolecular Tandem Assay
Lizeth Perez, Magi Mettry, Adam D. Gill, Richard J. Hooley, Yang Liu, Wenwan Zhong, Yinsheng Wang, Lin Li
Arrayed deep cavitands can be coupled to a fluorescence-based supramolecular tandem assay that allows site-selective in situ monitoring of post-translational modifications catalyzed by the lysine methyltransferase PRDM9 or the lysine demethylase JMJD2E. An arrayed sensor system containing only three cavitand components can detect the specific substrates of enzyme modification, in the presence of other histone peptides in the enzyme assay, enabling investigation of cross-reactivity over multiple methylation sites and interference from nonsubstrate peptides.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b05002

DOI: 10.1021/jacs.7b05002

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