3 years ago

An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase

An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase
Xing-Kai Qian, Yang Yu, Qun Luo, Tong-Yi Dou, Ling Yang, Li-Wei Zou, Shi-Yang Li, Dan-Dan Wang, Qiang Jin, Ping Wang, Yang-Liu Xia, Guang-Bo Ge
A practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes. A bright two-photon fluorescent probe 3-BTD for catechol-O-methyltransferase (COMT) is developed and characterized. 3-BTD can be used for real-time monitoring of COMT activities in complex biological samples and bio-imaging of endogenous COMT in living systems with good cell permeability, low cytotoxicity, and high imaging resolution.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201701384

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