3 years ago

A Single Composition Architecture-Based Nanoprobe for Ratiometric Photoacoustic Imaging of Glutathione (GSH) in Living Mice

A Single Composition Architecture-Based Nanoprobe for Ratiometric Photoacoustic Imaging of Glutathione (GSH) in Living Mice
Xiang Li, Chao Yin, Yufu Tang, Xiaozhen Li, Quli Fan, Jie Li, Zhen Yang, Wei Huang
As one of the reduction species, glutathione (GSH) plays a tremendous role in regulating the homeostasis of redox state in living body. Accurate imaging of GSH in vivo is highly desired to provide a real-time visualization of physiological and pathological conditions while it is still a big challenge. Recently developed photoacoustic imaging (PAI) with high resolution and deep penetration characteristics is more promising for in vivo GSH detection. However, its application is dramatically limited by the difficult designation of photoacoustic probes with changeable near-infrared (NIR)-absorption under reductive activation. A cyanine derivative-based activatable probe is developed for in vivo ratiometric PAI of GSH for the first time. The probe is structurally designed to output ratiometric signals toward GSH in NIR-absorption region based on the cleavage of disulfide bond followed by a subsequent exchange between the secondary amine and sulfydryl group formed. Such a ratiometric manner provides high signal-to-noise imaging of blood vessels and their surrounding areas in tumor. Concomitantly, it also exhibits good specificity toward GSH over other thiols. Furthermore, the single composition architecture of the probe effectively overcomes the leakage issue compared with traditional multicomposition architecture-based nanoprobe, thus enhancing the imaging accuracy and fidelity in living body. A cyanine derivative-based nanoprobe with specific glutathione (GSH) sensing capability is developed by taking advantage of the single composition architecture design, which effectively overcomes the dissociation-induced issues such as changed optical properties and unreliable imaging results compared with the multicomposition architecture-based nanoprobe fabricated from nanoprecipitation. This nanoprobe permits noninvasive real-time ratiometric photoacoustic imaging of GSH in tumor in living mice.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/smll.201703400

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