3 years ago

Thiol-triggered disaggregation-induced emission controlled by competitive coordination for acetylcholinesterase monitoring and inhibitor screening

Thiol-triggered disaggregation-induced emission controlled by competitive coordination for acetylcholinesterase monitoring and inhibitor screening
Acetylcholinesterase (AChE) is an important biomarker of many neurotransmitter-associated diseases and can be used to screen potential drugs and diagnose poisoning by chemical warfare agents and pesticides, and thus the accurate detection of AChE is of great practical significance. In this work, thiol-triggered disaggregation-induced emission through competitive interaction among 3,4,9,10-perylenetetracarboxylic acid (PTCA), copper ion and glutathione is identified, and a reliable fluorometric assay for acetylcholinesterase (AChE) activity is developed on the basis of this molecular switch. It is found that PTCA in neutral and alkaline solution exhibits very bright green fluorescence, and its quantum yield is up to 78% in alkaline solution. The presence of Cu2+ leads to the formation of PTCA/Cu(II) nanoaggregates and the quenching of the fluorescence, but the fluorescence can be fully switched on after the introduction of equivalent amount of thiol-containing compounds such as glutathione. A reversible fluorescence switch can be run in this way by the alternative introduction of Cu2+ and GSH. This switch enables the detection of AChE in the signal turn-on mode when acetylthiocholine is used as the substrate. Quantitative measurement of AChE level with detection limit as low as 4U/L and a broad linear scope ranging from 15 to 500.0U/L is achieved based on the developed assay. The inhibitor screening function was also assessed using tacrine as the example, and the assay performs well in the screening tacrine. This work proposed thiol-triggered disaggregation-induced emission mediated by competitive interaction, and provides a sensitive detection method for AChE level and its inhibitor screening.

Publisher URL: www.sciencedirect.com/science

DOI: S0925400517314661

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