3 years ago

Spatiotemporal Control of TGF-β Signaling with Light

Spatiotemporal Control of TGF-β Signaling
with Light
Guoyu Wu, Xuedong Liu, Minji Lee, Won Do Heo, Zhike Zi, Jin Man Kim, Difan Deng, Yuchao Li, Nury Kim
Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space. Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.

Publisher URL: http://dx.doi.org/10.1021/acssynbio.7b00225

DOI: 10.1021/acssynbio.7b00225

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