3 years ago

Dual-Emission Channels for Simultaneous Sensing of Cysteine and Homocysteine in Living Cells

Dual-Emission Channels for Simultaneous Sensing of Cysteine and Homocysteine in Living Cells
Pengfei Wang, Jiechao Ge, Mengqi Wang, Hongyan Zhang, Weimin Liu, Jiasheng Wu, Ying Li
The development of a fluorescent probe to distinguish between cysteine (Cys) and homocysteine (Hcy) is always a challenge owing to their structural similarity, and the simultaneous detection of Cys and Hcy by utilizing different emission channels is especially difficult. In this work, we designed and synthesized a new fluorescent probe to differentiate between Cys and Hcy on the basis of a coumarin derivative with a chlorine atom and an α,β-unsaturated aldehyde. Cys and Hcy induced different cascade reactions with the probe, which led to different products with distinct photophysical properties. The nonfluorescent probe responded to Cys and emitted strong blue fluorescence, whereas it reacted with Hcy and generated yellow fluorescence without interference from glutathione. In addition, the probe was successfully applied to distinguish between Cys and Hcy in living cells. Probing for answers: We constructed a new fluorescent probe to differentiate between cysteine (Cys) and homocysteine (Hcy) on the basis of different cascade reactions. The nonfluorescent probe responds to Cys and emits strong blue fluorescence, whereas it reacts with Hcy and generates yellow fluorescence without interference from glutathione. Fluorescent images indicate that the probe can distinguish between Cys and Hcy in living cells by utilizing different emission channels.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/asia.201700583

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