Chemistry - An Asian Journal
Chemistry - An Asian Journal
5 years ago

Polydiacetylene/Anti-HBs Complexes for Visible and Fluorescent Detection of Hepatitis B Surface Antigen on a Nitrocellulose Membrane

Polydiacetylene/Anti-HBs Complexes for Visible and Fluorescent Detection of Hepatitis B Surface Antigen on a Nitrocellulose Membrane
Dong June Ahn, Jinkyu Roh, Su Yeon Lee, Sangho Park
The immunochromatographic assay (ICA) using a nitrocellulose (NC) membrane offers several advantages. This technique is a rapid and straightforward method in contrast to other immunoassays. Polydiacetylene (PDA) vesicles have unique optical properties, displaying red color and red fluorescence at the same time. In this system, red-phase PDA vesicles are used as a fluorescent dye as well as a surface for immobilized hepatitis B surface antibody (HBsAb). PDA has a remarkable stability compared with other fluorescent dyes. In this study, the most suitable PDA/HBsAb complexes are introduced for detecting hepatitis B surface antigen (HBsAg). Then, the PDA/HBsAb complexes affixed antibody is attached to NC membrane, which has two lines to confirm detection of HBsAg. The main advantage of this system is that the detection of HBsAg can be observed in both visible and fluorescent images due to the optical properties of polydiacetylene. Detection of HBsAg is observed up to 0.1 ng mL−1 by fluorescent analysis and confirmed by red line on the NC membrane up to 1 ng mL−1 (HBsAg) using the naked eye. Consequently, these results show that PDA/HBsAb complexes were successfully applied to ICA for the diagnosis of hepatitis B. Visual detection of hepatitis B: Polydiacetylene (PDA)/hepatitis B surface antibody (PDA/HBsAb) complexes were introduced to an immunochromatographic assay, which can detect hepatitis B surface antigen (HBsAg) on a nitrocellulose (NC) membrane. Due to the ability of PDA to emit both visible and fluorescent radiation concurrently, the diagnostic results of hepatitis B were easily observed.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/asia.201700769

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