3 years ago

Highly parallel direct RNA sequencing on an array of nanopores

Highly parallel direct RNA sequencing on an array of nanopores
Sabrina Serra, Lakmal Jayasinghe, Tigist Admassu, Daniel J Turner, Botond Sipos, Daniel Jachimowicz, Sissel Juul, Chris Wright, Jemma Keenan, Joseph H Lloyd, James Clarke, Javier Blasco, Jonah Ciccone, Denise Brocklebank, Mark Bruce, Samuel Martin, Michael Jordan, Phillip James, E Jayne Wallace, Anthony Warland, Nadia Pantic, Stephen Young, Luke McNeill, Andrew J Heron, Daniel R Garalde, Elizabeth A Snell
Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.

Publisher URL: https://www.nature.com/articles/nmeth.4577

DOI: 10.1038/nmeth.4577

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