3 years ago

Mechanosensing drives acuity of {alpha}{beta} T-cell recognition [Biophysics and Computational Biology]

Mechanosensing drives acuity of {alpha}{beta} T-cell recognition [Biophysics and Computational Biology]
Yinnian Feng, Eiji Kobayashi, Matthew J. Lang, Robert J. Mallis, Kristine N. Brazin, Ellis L. Reinherz

T lymphocytes use surface αβαβ T-cell receptors (TCRs) to recognize peptides bound to MHC molecules (pMHCs) on antigen-presenting cells (APCs). How the exquisite specificity of high-avidity T cells is achieved is unknown but essential, given the paucity of foreign pMHC ligands relative to the ubiquitous self-pMHC array on an APC. Using optical traps, we determine physicochemical triggering thresholds based on load and force direction. Strikingly, chemical thresholds in the absence of external load require orders of magnitude higher pMHC numbers than observed physiologically. In contrast, force applied in the shear direction (10 pN per TCR molecule) triggers T-cell Ca2+ flux with as few as two pMHC molecules at the interacting surface interface with rapid positional relaxation associated with similarly directed motor-dependent transport via 8-nm steps, behaviors inconsistent with serial engagement during initial TCR triggering. These synergistic directional forces generated during cell motility are essential for adaptive T-cell immunity against infectious pathogens and cancers.

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