3 years ago

Intracellular vesicle trafficking plays an essential role in mitochondrial quality control.

Giuseppe Cannino, Mike Gerards, Jose M González de Cózar, Howard T Jacobs
The Drosophila gene products Bet1, Slh and CG10144, predicted to function in intracellular vesicle trafficking, were previously found to be essential for mitochondrial nucleoid maintenance. Here we show that Slh and Bet1 co-operate to maintain mitochondrial functions. In their absence, mitochondrial content, membrane potential and respiration became abnormal, accompanied by mitochondrial proteotoxic stress, but without direct effects on mtDNA. Immunocytochemistry showed that both Slh and Bet1 are localized at the Golgi, together with a proportion of Rab5-positive vesicles. Some Bet1, as well as a tiny amount of Slh, co-fractionated with highly purified mitochondria, whilst live-cell imaging showed coincidence of fluorescently tagged Bet1 with most Lysotracker-positive and a small proportion of Mitotracker-positive structures. This 3-way association was disrupted in cells knocked down for Slh, although co-localized lysosomal and mitochondrial signals were still seen. Neither Slh nor Bet1 were required for global mitophagy or endocytosis, but prolonged Slh knockdown resulted in G2 growth arrest, with increased cell diameter. These effects were shared with knockdown of betaCOP but not of CG1044, Snap24 or Syntaxin6. Our findings implicate vesicle sorting at the cis-Golgi in mitochondrial quality control.

Publisher URL: http://doi.org/10.1091/mbc.E17-10-0619

DOI: 10.1091/mbc.E17-10-0619

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