3 years ago

Activities of phospholipases A and lysophospholipases in glycolytic and oxidative skeletal muscles in the rabbit.

Corinne Alasnier, Gilles Gandemer
Oxidative muscles contain more free fatty acids than glycolytic muscles, which could explain in part their higher sensitivity to oxidation. These fatty acids are partly the result of phospholipid hydrolysis catalysed by phospholipases A and lysophospholipases. Up to now, very little is known on the activities of these enzymes in skeletal muscles. This study deals with the activities of phospholipases A and lysophospholipases in five rabbit muscles covering a large range of metabolic types (oxidative Soleus and Semimembranosus proprius muscles, glycolytic Psoas major and Longissimus lumborum muscles and intermediate Gastrocnemius laterale muscle). The results showed that (a) phospholipases A and lysophospholipases had maximal activity at pH 8-9; (b) phospholipases A and lysophospholipases retained more than 50% of their maximal activity at pH 5.5-6, the ultimate pH of muscles; (c) lysophospholipases exhibited a higher activity than phospholipases A (4-7-fold higher in the oxidative muscles, 11-fold higher in the intermediate muscle and 18-23-fold higher in the glycolytic muscles); and (d) phospholipase A and lysophospholipase activities were higher in oxidative muscles than in glycolytic muscles (10-25-fold higher for phospholipases A and 4-5-fold higher for lysophospholipases). Thus oxidative muscles have a higher potential activity for post-mortem hydrolysis of phospholipids. © 2000 Society of Chemical Industry.

Publisher URL: http://doi.org/10.1002/(SICI)1097-0010(20000501)80:6<698::AID-JSFA591>3.0.CO;2-J

DOI: 10.1002/(SICI)1097-0010(20000501)80:6<698::AID-JSFA591>3.0.CO;2-J

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.