3 years ago

An arrayed Micro-Glutamate Sensor Probe Integrated with On-Probe Ag/AgCl Reference and Counter Electrodes

An arrayed Micro-Glutamate Sensor Probe Integrated with On-Probe Ag/AgCl Reference and Counter Electrodes
Hong-Ru Chen, Tina T.-C. Tseng, Le Ngoc Quynh Hoa
Complete all-in-one multi-arrayed glutamate (Glut) sensors have been constructed on a silicon-based micromachined probe composed of micro-platinum (Pt) working electrodes, a micro-silver/silver chloride (Ag/AgCl) reference electrode (RE), and a micro-Pt counter electrode (CE). The OCP shift of the electrodeposited Ag/AgCl on-probe micro-reference electrode compared with a Ag/AgCl wire is <0.1 mV/h. The composition ratio of Ag, Cl, and Pt on the electrodeposited on-probe micro-reference electrode is observed to be 1.00 : 0.48 : 0.02 analyzed by EDS. The miniaturized amperometric Glut biosensors were constructed on working electrode sites (electrode area: ∼8.5×10−5 cm2) of the microprobe modified with glutamate oxidase (GlutOx) enzyme layers for the selective, fast, and continuous detection of L-glutamate. The sensor selectivity towards common electroactive interferents has been improved significantly by coating the electrode surface with perm-selective polymer layers, overoxidized polypyrrole (PPY) and Nafion®. The sensitivity, detection range, and response time of the proposed all-in-one Glut biosensors are 204.7±5.8 nA μM−1 cm−2 (N=5), 4.99–109 μM, and 2.7±0.3 sec, respectively and no interferent signals of AA and DA were observed. The sensor can be reused over 19 times of continuous repetitive operation (total measurement time: ∼4 hours) and the sensor sensitivity can retain up to four weeks of storage.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/elan.201700762

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