5 years ago

Does Surface Chirality of Gold Nanoparticles Affect Fibrillation of HSA?

Does Surface Chirality of Gold Nanoparticles Affect Fibrillation of HSA?
Shubhatam Sen, Sunando DasGupta, Swagata Dasgupta
In order to demonstrate the influence of the surface chirality of the nanoparticles on amyloid fibrillation, the inhibiting effectiveness of the chiral gold nanoparticles, synthesized using the two enantiomeric forms (i.e., d- and l-) of glutamic acid, toward the fibrillation of human serum albumin (HSA) has been investigated. Here the enantiomers of glutamic acid are used as both reducing and stabilizing/capping agents. It is found that the surface chirality is the only major difference between the d-glutamic acid mediated gold (DGAu) and l-glutamic acid mediated gold (LGAu) nanoparticles. The fibrillation process has been monitored using various biophysical techniques, e.g., turbidity assay, Thioflavin T fluorescence kinetics, Congo red binding study, circular dichroism spectroscopy, fluorescence microscopy, and transmission electron microscopy. The experimental results illustrate that DGAu is more effective in inhibiting the formation of HSA fibrils than LGAu. Furthermore, the differential inhibiting effect of DGAu and LGAu toward HSA fibrillation has been described on the basis of the dissimilar interaction behavior of the nanoparticles with the HSA molecules, as revealed by the Trp fluorescence quenching, and the isothermal titration calorimetry. It is suggested that the surface chirality of the gold nanoparticles strongly influences the protein adsorption dynamics including the modes of orientation of adsorbed HSA molecules, causing the inhibition of fibrillation to different extents.

Publisher URL: http://dx.doi.org/10.1021/acs.jpcc.7b05354

DOI: 10.1021/acs.jpcc.7b05354

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.