3 years ago

Characterization of a novel RP2-OSTF1 interaction and its implication for actin remodeling.

Mandy Lokaj, Rodanthi Lyraki, Joseph A Marsh, Alfred Wittinghofer, Abigail Little, Matthieu Vermeren, Toby Hurd, Dinesh C Soares
Retinitis pigmentosa 2 (RP2) is the causative gene for a form of X-linked retinal degeneration. RP2 was previously shown to have GAP activity towards the small GTPase ARL3 via its N-terminus, but the function of the C-terminus remains elusive. Here, we report a novel interaction between RP2 and Osteoclast-stimulating factor 1 (OSTF1), an intracellular protein that indirectly enhances osteoclast formation and activity and is a negative regulator of cell motility. Moreover, this interaction is abolished by a human pathogenic mutation in RP2. We utilized a structure-based approach to pinpoint the binding interface to a strictly conserved cluster of residues on the surface of RP2 that spans both the C- and N- terminal domains of the protein, and which is structurally distinct from the ARL3 binding site. In addition, we show that RP2 is a positive regulator of cell motility in vitro, recruiting OSTF1 to the cell membrane and preventing its interaction with the migration regulator Myo1E.

Publisher URL: http://doi.org/10.1242/jcs.211748

DOI: 10.1242/jcs.211748

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