Organic Letters
Organic Letters
5 years ago

Superior Fluorogen-Activating Protein Probes Based on 3-Indole–Malachite Green

Superior Fluorogen-Activating Protein Probes Based on 3-Indole–Malachite Green
Verena Fetz, Ying Sun, Limin Zuo, Qinghua Wang, Qingyang Zhang, Hai-Yu Hu
A series of novel fluorogenic dyes based on 3-indole–Malachite Green, MGs 5–7, have been developed that are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP). Significantly, the new MGs 5–7 probes are superior to the traditional MG 1 with high fluorescent efficiency and low toxicity to cells while maintaining the large “pseudo-Stokes” shifts (Δλ = λex – λem) and the malachite green (MG)-like fluorescence OFF–ON switching mechanism in both live mammalian cells and bacterial cells when bound to FAP.

Publisher URL: http://dx.doi.org/10.1021/acs.orglett.7b02055

DOI: 10.1021/acs.orglett.7b02055

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