3 years ago

Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness.

Lana Shabala, Qinsong Liu, Victoria Sanchez-Vera, Sergey Shabala, Ondrej Novák, Kerstin Dalman, Karin Ljung, Sten Stymne, Peter V Bozhkov, Catarina Cardoso, Jenny Lindberg Yilmaz, Maria F Suarez, Ramesh R Vetukuri, Daniel Hofius, Mirela Beganovic, Panagiotis N Moschou, Elena A Minina, Pernilla H Elander, Sofia Marmon
Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic compartment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5 and Atg8 with lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these systems causes autophagy-deficient phenotypes with reduced fitness and longevity. We show that Atg5 and E1-like enzyme, Atg7, are rate-limiting components of the Atg8-PE conjugation in Arabidopsis. Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation and autophagic flux. It also induces transcriptional changes opposite to those observed in atg5 and atg7 mutants, favoring stress resistance and growth. As a result, ATG5- or ATG7-overexpressing plants exhibit increased resistance to necrotrophic pathogens and oxidative stress, delayed aging and enhanced growth, seed set and seed oil content. This work provides experimental paradigm and mechanistic insight into genetic stimulation of autophagy in planta and shows its efficiency for improving plant productivity.

Publisher URL: http://doi.org/10.1093/jxb/ery010

DOI: 10.1093/jxb/ery010

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.