3 years ago

N-carboxyanhydride polymerization of glycopolypeptides that activate antigen presenting cells through Dectin-1 and -2

Justin A Kenkel, Edgar G Engleman, Jessica R Kramer, Carolyn R. Bertozzi, Matthew Zhou, Corleone S Delaveris
The C-type lectins Dectin-1 and Dectin-2 contribute to innate immunity against microbial pathogens by recognizing their foreign glycan structures. These receptors are promising targets for immune stimulatory adjuvants in the context of vaccine development and cancer immune therapy. However, currently available agonist ligands are heterogeneous glycoconjugates and polysaccharides from natural sources. Here, we designed and synthesized the first chemically-defined ligands for Dectin-1 and -2. They comprised glycopolypeptides bearing mono-, di-, and trisaccharides and were built via polymerization of glycosylated N-carboxyanhydrides. Through this approach, we achieved glycopolypeptides with high molecular weights and low dispersities. We identified structures that elicit a proinflammatory response through Dectin-1 or -2 in antigen presenting cells. With their native proteinaceous backbones and natural glycosidic linkages, these agonists are attractive for translational applications.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201713075

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