3 years ago

Freshness assessment of intact fish via 2D 1H J-resolved NMR spectroscopy combined with pattern recognition methods

Freshness assessment of intact fish via 2D 1H J-resolved NMR spectroscopy combined with pattern recognition methods
Freshness of intact zebrafish was evaluated by 2D 1H J-resolved NMR spectroscopy conjugated with pattern recognition methods Without complicated sample pretreatment and specialized hardware settings, the biochemical processes of intact zebrafish from fresh to decay state and the corresponding metabolite profiles were acquired. A total of 21 metabolites were identified and their concentration variations during the spoilage process were obtained. Pattern recognition models were established for discrimination of fish from freshness to decomposition status. The results show that the concentration of lipid declines, the concentration of lactate fluctuates, and the concentrations of glycerol, asparagine, sarcosine, tyrosine, acetate, glycine and serine increase over the storage time. The observed acetate accumulation and lactate consumption may be considered as indexes for fish freshness assessment. This study indicates that the combination of high-resolution 2D 1H J-resolved NMR spectroscopy and pattern recognition methods may provide an efficient way for quality evaluation of semisolid and viscous foods.

Publisher URL: www.sciencedirect.com/science

DOI: S092540051731482X

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