3 years ago

Bioavailability of mineral-bound iron to a snow algae-bacteria co-culture and implications for albedo-altering snow algae blooms.

A E Murray, E M Hausrath, O Tschauner, A H Garcia, S Huang, J Raymond, Z R Harrold
Snow algae can form large-scale blooms across the snowpack surface and near-surface environments. These pigmented blooms can decrease snow albedo, increase local melt rates, and may impact the global heat budget and water cycle. Yet, underlying causes for the geospatial occurrence of these blooms remain unconstrained. One possible factor contributing to snow algae blooms is the presence of mineral dust as a micronutrient source. We investigated the bioavailability of iron (Fe) -bearing minerals, including forsterite (Fo90, Mg1.8Fe0.2SiO4), goethite, smectite and pyrite as Fe sources for a Chloromonas brevispina - bacteria co-culture through laboratory-based experimentation. Fo90 was capable of stimulating snow algal growth and increased the algal growth rate in otherwise Fe-depleted co-cultures. Fo90-bearing systems also exhibited a decrease in bacteria:algae ratios compared to Fe-depleted conditions, suggesting a shift in microbial community structure. The C. brevispina co-culture also increased the rate of Fo90 dissolution relative to an abiotic control. Analysis of 16S rRNA genes in the co-culture identified Gammaproteobacteria, Betaprotoeobacteria and Sphingobacteria, all of which are commonly found in snow and ice environments. Archaea were not detected. Collimonas and Pseudomonas, which are known to enhance mineral weathering rates, comprised two of the top eight (> 1 %) OTUs. These data provide unequivocal evidence that mineral dust can support elevated snow algae growth under otherwise Fe-depleted growth conditions, and that snow algae can enhance mineral dissolution under these conditions.IMPORTANCE Fe, a key micronutrient for photosynthetic growth, is necessary to support the formation of high-density snow algae blooms. The laboratory experiments described herein allow for a systematic investigation of snow algae-bacteria-mineral interactions and their ability to mobilize and uptake mineral-bound Fe. Results provide unequivocal and comprehensive evidence that mineral-bound Fe in Fe-bearing Fo90 was bioavailable to Chloromonas brevispina snow algae within an algae-bacteria co-culture. This evidence includes: 1) an observed increase snow algae density and growth rate; 2) decreased bacteria:algae ratios in Fo90-containing cultures relative to cultures grown under similarly Fe-depleted conditions with no mineral-bound Fe present; and 3) increased Fo90 dissolution rates in the presence of algae-bacteria co-cultures relative to abiotic mineral controls. These results have important implications for the role of mineral dust in supplying micronutrients to the snow microbiome, which may help support dense snow algae blooms capable of lowering snow albedo, and increase snow melt rates on regional, and possibly global, scales.

Publisher URL: http://doi.org/10.1128/AEM.02322-17

DOI: 10.1128/AEM.02322-17

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