3 years ago

Biophotonics of Native Silk Fibrils

Biophotonics of Native Silk Fibrils
Christopher M. Dobson, Dorothea Pinotsi, Klimentiy Shimanovich, Chris Holland, Clemens F. Kaminski, Jerome Charmet, Ehud Gazit, Gabriele Kaminski Schierle, Jozef Adamcik, Ulyana Shimanovich, Tuomas P. J. Knowles, Raffaele Mezzenga, Sreenath Bolisetty, Fritz Vollrath, Na Yu
Native silk fibroin (NSF) is a unique biomaterial with extraordinary mechanical and biochemical properties. These key characteristics are directly associated with the physical transformation of unstructured, soluble NSF into highly organized nano- and microscale fibrils rich in β-sheet content. Here, it is shown that this NSF fibrillation process is accompanied by the development of intrinsic fluorescence in the visible range, upon near-UV excitation, a phenomenon that has not been investigated in detail to date. Here, the optical and fluorescence characteristics of NSF fibrils are probed and a route for potential applications in the field of self-assembled optically active biomaterials and systems is explored. In particular, it is demonstrated that NSF can be structured into autofluorescent microcapsules with a controllable level of β-sheet content and fluorescence properties. Furthermore, a facile and efficient fabrication route that permits arbitrary patterns of NSF microcapsules to be deposited on substrates under ambient conditions is shown. The resulting fluorescent NSF patterns display a high level of photostability. These results demonstrate the potential of using native silk as a new class of biocompatible photonic material. In this work it is demonstrated that native silk fibroin (NSF) fibrillation process is accompanied by, and directly linked to the development of intrinsic fluorescence in the visible range, upon near-UV excitation. The aggregation-induced optical characteristics of silk together with its biodegradability and biocompatibility may open the door to the development of a new class of functional and fluorescent biomaterials.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mabi.201700295

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.