Chemistry - A European Journal
Chemistry - A European Journal
5 years ago

Substitution of an Internal Disulfide Bridge with a Diselenide Enhances both Foldability and Stability of Human Insulin

Orit Weil‐Ktorza, Nischay Rege, Shifra Lansky, Deborah E. Shalev, Gil Shoham, Michael A. Weiss, Norman Metanis
Chemical preparation of insulin is challenging due to the two‐chain (designated A and B) structure of this hormone, which exhibits a specific pattern of disulfide pairing. Traditional methods of synthesis, based on chain combination, are limited by slow and inefficient disulfide pairing. This limitation can be overcome by use of a modified A chain, in which cysteine residues at positions 6 and 11 are replaced with selenocysteines. The product seleno‐insulin (containing an internal A6–A11 diselenide bond) was rapidly obtained in high yield. The analogue retains native binding to the insulin receptor and exhibits augmented stability. More information can be found in the Full Paper by M. A. Weiss, N. Metanis et al. (DOI: 10.1002/chem.201900892).

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201901973

Open URL: https://onlinelibrary.wiley.com/doi/pdf/10.1002/chem.201901973

DOI: 10.1002/chem.201901973

Open access
You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.