5 years ago

Native Collagen and Its Structural Fulcrum through a Site Specific Hydration Topology Map

Native Collagen and Its Structural Fulcrum through a Site Specific Hydration Topology Map
Neeraj Sinha, Akhila Viswan
Structural account of collagen in its native environment is limited, and a water-mediated hydrogen bonding network as its stability analogue needs to be contemplated site specifically. We present in this Article that, through natural abundance 13C chemical shift anisotropy (CSA) measurement of collagen in its native state (inside bone matrix), structural and mechanistic insight of the water-mediated hydrogen bonding network can be achieved. The 13C CSA of backbone and side chain residues of collagen, perturbed under dehydration and H/D exchange conditions, can be measured. The changes in 13C CSA values due to perturbation of water content have resulted in determining the site-specific accessibility of water molecules. A further hydration topology map (HTM) representing water accessibility of native collagen has been generated on the basis of changes in 13C CSA values. Our results signify the water accessibility of the proline/hydroxyproline carbonyl region is larger compared to the hydroxyproline Cγ site and open up new avenues for collagen structure determination in its native environment.

Publisher URL: http://dx.doi.org/10.1021/acs.jpcc.7b05185

DOI: 10.1021/acs.jpcc.7b05185

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.