Enhanced rhamnolipid production by <i>Pseudomonas aeruginosa</i> overexpressing <i>estA</i> in a simple medium
by Leticia Dobler, Bruna Rocha de Carvalho, Wilber de Sousa Alves, Bianca Cruz Neves, Denise Maria Guimarães Freire, Rodrigo Volcan Almeida
A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML) production was studied. Fermentations using wild-type (WT) and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L RML when measured by HPLC and 22 g/L by the orcinol assay. Cell-free supernatant from the modified strain reduced surface tension to 29.4 mN/m and had a CMC of 240 mg/L and CMD of 56.05. This is the first report on the construction of an estA-based recombinant strain for RML production.Publisher URL: http://journals.plos.org/plosone/article
DOI: 10.1371/journal.pone.0183857
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