4 years ago

Enhanced rhamnolipid production by <i>Pseudomonas aeruginosa</i> overexpressing <i>estA</i> in a simple medium

Rodrigo Volcan Almeida, Bianca Cruz Neves, Wilber de Sousa Alves, Denise Maria Guimarães Freire, Bruna Rocha de Carvalho, Leticia Dobler

by Leticia Dobler, Bruna Rocha de Carvalho, Wilber de Sousa Alves, Bianca Cruz Neves, Denise Maria Guimarães Freire, Rodrigo Volcan Almeida

A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML) production was studied. Fermentations using wild-type (WT) and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L RML when measured by HPLC and 22 g/L by the orcinol assay. Cell-free supernatant from the modified strain reduced surface tension to 29.4 mN/m and had a CMC of 240 mg/L and CMD of 56.05. This is the first report on the construction of an estA-based recombinant strain for RML production.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.pone.0183857

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