Pest Management Science
Pest Management Science
5 years ago

Larvicidal efficacy of Adiantobischrysene from Adiantum latifolium against Oryctes rhinoceros through disrupting metamorphosis and impeding microbial mediated digestion

K.V Dinesh Babu, Anil John, D.A Evans, Praveen Kumar, R Pradeep Kumar
Background Oryctes rhinoceros Linn. (Coleoptera: Scarabaeidae) is one of the serious pests of coconuts and other palms. Symbiotic gut bacteria play significant roles in digestion of cellulosic materials as well as in some other physiological processes essential for the existence of the O. rhinoceros larvae. The study was undertaken to isolate compound with antibacterial and larvicidal activities from the leaves of Adiantum latifolium Lam. following bioassay-guided method. Result Methanol extract (ME) of dry leaf powder of A. latifolium showed larvicidal activity against third instar of O. rhinoceros (LD50 value 5018mg/kg) with antibacterial activity on its gut microbiota. In vitro study showed all the bacteria, Bacillus cereus, Micrococcus lylae, Stenotrophomonas maltophilia, Kocuria rosea, Burkholderia mallei, Staphylococcus epidermidis, S. arlettae and Corynebacterium afermentans, identified from the larval gut were sensitive to ME . Bioactivity guided isolation of compound by liquid-liquid extraction and column chromatography resulted in Adiantobischrysene proved for its antibacterial and larvicidal activity (LD50 value 8.4mg/kg), weight loss and precocious metamorphosis of larvae. Enzyme immunoassay showed large peak in 20-hydroxyecdysone that commits larvae to precocious metamorphosis. Conclusion The present study demonstrated that antibacterial and metamorphosis disrupting activity of Adiantobischrysene impart it to be used as a natural pesticidal compound against O. rhinoceros.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/ps.4880

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