Analytical Chemistry
Analytical Chemistry
5 years ago

Inkjet Printing Based Separation of Mammalian Cells by Capillary Electrophoresis

Inkjet Printing Based Separation of Mammalian Cells by Capillary Electrophoresis
Hizuru Nakajima, Weifei Zhang, Nan Li, Katsumi Uchiyama, Hulie Zeng, Jin-Ming Lin
This study describes a method to investigate the separation of cells by capillary electrophoresis (CE) coupled with inkjet printing system. The results validated the feasibility of inkjet printing for mammalian cells to achieve the drop-on-demand and convenient sampling into capillary then zone electrophoresis was applied to separate different cells according to their electrophoretic mobility, finally the peak signal were measured by UV detector. Linear relationship between the peak area and the droplet number was obtained within the range of 25–400 drops (R2 = 0.996) at a fixed cell concentration 106/mL, indicating that this system could be used for rapid and accurate quantification of cells.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b02624

DOI: 10.1021/acs.analchem.7b02624

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