ACS Chemical Biology
ACS Chemical Biology
5 years ago

Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae

Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae
Takayuki Motoyama, Hiroyuki Osada, Choong-Soo Yun
Tenuazonic acid (TeA) is a mycotoxin produced by the rice blast fungus Pyricularia oryzae and some plant pathogenic fungi. We previously demonstrated that TeA is biosynthesized in P. oryzae by TeA synthetase 1 (TAS1) and that its production is induced by osmo-sensory MAPK-encoding gene (OSM1) deletion or the addition of 1% DMSO to cultures; however, the regulatory mechanisms of TeA production were unknown. Here, we identify a Zn(II)2-Cys6-type transcription factor in the upstream region of TAS1, which is encoded by TAS2 and regulates TeA production. We also find PoLAE1, which is a homologue of LaeA, a regulator of fungal secondary metabolism. Analysis of PoLAE1 deletion and overexpression strains indicate that PoLAE1 drives TeA production. We also demonstrate that two TeA-inducing signals, 1% DMSO addition and OSM1 deletion, were transmitted through PoLAE1. Our results indicate that TeA production is regulated by two specific regulators, TAS2 and PoLAE1, in P. oryzae.

Publisher URL: http://dx.doi.org/10.1021/acschembio.7b00353

DOI: 10.1021/acschembio.7b00353

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