Clinical Microbiology and Infection
Clinical Microbiology and Infection
5 years ago

Evaluation of HiberGene Loop-Mediated Isothermal Amplification Assay for Detection of Group B Streptococcus in Recto-Vaginal swabs: A Prospective Diagnostic Accuracy Study

To prospectively evaluate HiberGene’s loop-mediated isothermal amplification (LAMP) assay for detection of Group B Streptococcus (GBS) in maternal recto-vaginal swabs and compare it to enrichment culture. Methods Following ethical approval and informed written consent, two low vaginal and rectal swabs were obtained from 400 pregnant women. One swab was tested for GBS using the rapid LAMP assay (index test), the second swab was tested using enrichment culture (reference standard). Antimicrobial susceptibility testing was performed according to EUCAST guidelines. Results There were 376 concordant results, 20 discordant and four invalid LAMP results. Among discordant results, six were LAMP negative / culture positive and 14 were LAMP positive / culture negative. The sensitivity was 92.2%, specificity 95.6%, positive predictive value 83.5% and negative predictive value 98.1%. The prevalence of GBS carriage was 19.25% (77/400). Forty-eight of 77 GBS positive women were colonised vaginally (62.3%) and 70 were colonised rectally (90.9%). Erythromycin resistance was 22.4% (17/76) and clindamycin resistance 17.1% (13/76). Conclusions The LAMP assay is a rapid and simple test with results available in approximately one hour compared with 48 hours for culture. The test has good sensitivity and specificity compared to enrichment culture. This test can be used for rapid antenatal GBS screening.

Publisher URL: www.sciencedirect.com/science

DOI: S1198743X18300624

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