5 years ago

Antibiotic-affinity strategy for bioluminescent detection of viable Gram-positive bacteria using daptomycin as recognition agent

Antibiotic-affinity strategy for bioluminescent detection of viable Gram-positive bacteria using daptomycin as recognition agent
A bioluminescent method was proposed for rapid detection of viable Gram-positive bacteria based on a novel antibiotic-affinity strategy on a magnetic beads (MBs) platform. Daptomycin, a highly efficient lipopeptide antibiotic for Gram-positive bacteria, was used as a recognition agent to functionalize MBs. The daptomycin-functionalized MBs showed high capture and concentration efficiency for Gram-positive bacteria due to the strong binding between daptomycin and bacterial cell membrane in the presence of Ca2+ ion. The captured bacteria were lysed by hexadecyl trimethyl ammonium bromide solution, followed by a bioluminescent detection of the released intracellular adenosine triphosphate. Four Gram-positive bacteria, including Staphylococcus aureus, Streptococcus mutans, Bacillus subtilis and Staphylococcus epidermidis, were detected as model bacteria by this method. Under the optimal conditions, the bacteria could be detected within a linear range of 1.0 × 102–3.0 × 106 CFU mL−1, with a detection limit of 33 CFU mL−1. The whole detection procedure could be completed within 20 min. Gram-negative bacteria and dead Gram-positive bacteria showed negligible interference to the detection of viable Gram-positive bacteria. The proposed method was successfully applied to quantify the amount of viable Gram-positive bacteria in cheese, milk, lake water, human urine and physiological saline injection with acceptable recovery values ranging from 75.0% to 120.0%. The strategy possessed some advantages such as high sensitivity, short assay time and simple operation, thus showed great promise for food hygiene, environment monitoring, clinical diagnosis and drug safety.

Publisher URL: www.sciencedirect.com/science

DOI: S0003267017309674

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.