3 years ago

A review of the role of chemical modification methods in contemporary mass spectrometry-based proteomics research

A review of the role of chemical modification methods in contemporary mass spectrometry-based proteomics research
For more than two decades, mass spectrometry has been a central technology for proteomics research. During this time, the use of chemical reactions to introduce tags for quantification, affinity enrichment and other uses has facilitated the comprehensive profiling of the proteome by mass spectrometry in many ways. In the last decade, the introduction of more powerful instruments and more sophisticated data acquisition and data analysis routines has made some strategies obsolete, while making other approaches practically feasible only now. Here, the current status of chemical tagging strategies in proteomics is reviewed, with a particular emphasis on proteome quantification workflows, strategies for the enrichment of (post-translationally) modified proteins and peptides, and methods for structural proteomics.

Publisher URL: www.sciencedirect.com/science

DOI: S0003267017309637

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.