5 years ago

ROS mediated selection for increased NADPH availability in Escherichia coli

ROS mediated selection for increased NADPH availability in Escherichia coli
Keesha E. Erickson, Lisa M. Wolfe, Colleen M. Courtney, Ryan T. Gill, Anushree Chatterjee, Thomas S. Reynolds, Prashant Nagpal
The economical production of chemicals and fuels by microbial processes remains an intense area of interest in biotechnology. A key limitation in such efforts concerns the availability of key co-factors, in this case NADPH, required for target pathways. Many of the strategies pursued for increasing NADPH availability in Escherichia coli involve manipulations to the central metabolism, which can create redox imbalances and overall growth defects. In this study we used a reactive oxygen species based selection to search for novel methods of increasing NADPH availability. We report a loss of function mutation in the gene hdfR appears to increase NADPH availability in E. coli. Additionally, we show this excess NADPH can be used to improve the production of 3HP in E. coli. An ROS based selection was used to find mutations in E. coli that increased NADPH availability. These adapted cells were then found to increase production of 3HP.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/bit.26385

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