Fermentation strategy for second generation ethanol production from sugarcane bagasse hydrolyzate by Spathaspora passalidarum and Scheffersomyces stipitis

5 years ago

Fermentation strategy for second generation ethanol production from sugarcane bagasse hydrolyzate by Spathaspora passalidarum and Scheffersomyces stipitis

Aline C. Costa, Simone C. Nakanishi, George Jackson M. Rocha, Viviane M. Nascimento, Lauren B. Soares, Luiz Eduardo Biazi, Jaciane L. Ienczak

Alcoholic fermentation of released sugars in pretreatment and enzymatic hydrolysis of biomass is a central feature for second generation ethanol (E2G) production. Saccharomyces cerevisiae used industrially in the production of first generation ethanol (E1G) convert sucrose, fructose, and glucose into ethanol. However, these yeasts have no ability to ferment pentose (xylose). Therefore, the present work has focused on E2G production by Scheffersomyces stipitis and Spathaspora passalidarum. The fermentation strategy with high pitch, cell recycle, fed-batch mode, and temperature decrease for each batch were performed in a hydrolyzate obtained from a pretreatment at 130°C with NaOH solution (1.5% w/v) added with 0.15% (w/w) of anthraquinone (AQ) and followed by enzymatic hydrolysis. The process strategy has increased volumetric productivity from 0.35 to 0.38 g · L−1 · h−1 (first to third batch) for S. stipitis and from 0.38 to 0.81 g · L−1 · h−1 for S. passalidarum (first to fourth batch). Mass balance for the process proposed in this work showed the production of 177.33 kg ethanol/ton of sugar cane bagasse for S. passalidarum compared to 124.13 kg ethanol/ton of sugar cane bagasse for S. stipitis fermentation. The strategy proposed in this work can be considered as a promising strategy in the production of second generation ethanol. Biotechnol. Bioeng. 2017;9999: 1–11. © 2017 Wiley Periodicals, Inc. Fermentation strategies employed in this study as cell recycle, fed-batch mode and temperature decrease for each batch, performed in a hydrolyzate obtained from alkaline pretreatment of sugarcane bagasse followed by enzymatic hydrolysis increased volumetric productivity, favored xylose consumption and increased ethanol production. Mass balance showed production of 177.33 kg ethanol/ton of sugar cane bagasse for S. passalidarum and 124.13 kg ethanol/ton of sugar cane bagasse for S. stipitis fermentation. The strategy proposed can be considered a promising strategy for second generation ethanol production.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/bit.26357