Scientific Reports
Scientific Reports
5 years ago

Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane

Porntip Pan-In, Kittima Amornwachirabodee, Promchat Charoenpat, Pannamthip Pitaksajjakul, Patcharee Ritprajak, Chonlatip Pipattanaboon, Nattapol Tantimekin, Thanyada Sukmanee, Tanapat Palaga, Supason Wanichwecharungruang, Pongrama Ramasoota, Prompong Pienpinijtham
Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells’ interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies.

Publisher URL: https://www.nature.com/articles/s41598-018-20650-4

DOI: 10.1038/s41598-018-20650-4

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