Process Biochemistry
Process Biochemistry
5 years ago

Purification and identification of a novel peptide derived from by-products fermentation of spiny head croaker (Collichthys lucidus) with antifungal effects on phytopathogens

Purification and identification of a novel peptide derived from by-products fermentation of spiny head croaker (Collichthys lucidus) with antifungal effects on phytopathogens
In our previous study, spiny head croaker (Collichthys lucidus) by-products fermentation (SBF) products demonstrated plant growth promoting effects. In this study, the antimicrobial activities of SBF against plant pathogenic fungi and food-borne bacteria were evaluated. An active antifungal fraction SBF-3-1 was isolated from SBF using size exclusion chromatography and high performance liquid chromatography. Seven short peptides, i.e., LDEGW, DSFDFK, LDGW, FPDL, YNLDFK, FDGF and LDFE, plus one 22 residues peptide TFNTPAMYVAIQAVLSLYASGR were identified in SBF-3-1 by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS). The peptide TFNTPAMYVAIQAVLSLYASGR (named as SBF-3-1p), a potential antimicrobial peptide predicted by the antimicrobial peptide database (APD, http://aps.unmc.edu/AP/main.php), was further characterized. The prediction showed that the generation of SBF-3-1p could be associated with the action of trypsin and glutamyl endopeptidase, two proteases with specific cleavage sites on the carboxyl side of arginine (R) and lysine (K), and on the carboxyl side of Glu (E), respectively. According to the structural modeling, SBF-3-1p contained one α-helix and two loop regions. The synthesized SBF-3-1p exhibited strong activity against plant pathogenic fungus Pestalotiopsis sp. Therefore, SBF should be a good source of antifungal peptide, and could be used as natural fungicides in agriculture.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511317304439

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.