4 years ago

Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid

Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid
Poly(ethylene terephthalate) (PET) is one of the most important industrial plastics in the world. The development of technologies for its depolymerization aiming at monomer recycling is of paramount interest and in this context enzyme-catalyzed hydrolysis has been recognized as a promising alternative. In the present study, a screening of 10 commercial enzymes using bis-(hydroxyethyl) terephthalate (BHET) as model substrate revealed Candida antarctica lipase B (CALB) and Humicola insolens cutinase (HiC) as potential biocatalysts. HiC showed limitation for the last reaction step, but CALB completely converted BHET to terephthalic acid (TPA), even at the highest substrate concentration investigated (31mM). When used for PET hydrolysis, HiC demonstrated better potential, although accumulating considerable amounts of the intermediate mono-(hydroxyethyl) terephthalate (MHET). After evaluation of the effects of PET pretreatment, temperature and enzyme addition strategy, the combination of these two enzymes revealed synergy for a more complete PET depolymerization to TPA (mole fraction up to 0.88) and a 7.7–fold increase in PET to TPA yield was achieved. This finding adds new investigation possibilities for one of the most studied and versatile lipases, CALB.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511316302720

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.