Process Biochemistry
Process Biochemistry
5 years ago

S-Adenosyl-l-methionine production by Saccharomyces cerevisiae SAM 0801 using dl-methionine mixture: From laboratory to pilot scale

S-Adenosyl-l-methionine production by Saccharomyces cerevisiae SAM 0801 using dl-methionine mixture: From laboratory to pilot scale
This study sought to develop a cost-effective biological catalysis process for S-adenosyl-l-methionine (SAM) production. During the process, a mixture of d-methionine (d-Met) and l-methionine (l-Met), namely dl-Met, was used as substrate to replace the conventional but expensive pure l-Met. The concentration of dl-Met in substrate was optimized. When 80g/L of dl-Met was added in a 5L-scale bioreactor, 13.74g/L of SAM was produced with an l-Met conversion rate of 32.15%. The fermentation process was then scaled up to meet the requirements of realistic industrial SAM production. In a 300L-scale fermentation process, 10.45g/L of SAM was achieved, with an l-Met conversion rate of 32.61%. Moreover, the proportion of d-Met remaining in broth increased from 50% at the beginning to 76.89% at the end of fermentation. The fermentation process is generally appropriate for commercial SAM production.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511317304506

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