Using electropolymerization based doping for electro-addressable functionalization of a multi-electrode array probe for nucleic acid detection.

Here, we report a facile method for electro-addressable functionalization of a probe comprising of closely spaced three individually addressable carbon fiber electrodes for detection of nucleic acids. First, a multi electrode array probe comprising three adjacent carbon fiber electrodes was fabricated through pulling a three-barrel glass capillary with a single carbon fiber in each barrel using a micropuller. Second, electropolymerization based doping was used for electro-addressable functionalization of the multi-electrode array probe. To demonstrate that the current strategy works, anti-miR-34a was electrografted on only one of three electrodes by electropolymerization of pyrrole on the specific electrode. A second electrode was coated only with polypyrrole (PPy) and the third was left unmodified. Electrochemical impedance spectroscopy (EIS) was used for analysis and the results clearly showed charge transfer resistance of the PPy + anti-miR-34a modified electrode increased significantly after hybridization, while charge transfer resistance of the other two electrodes remained almost constant. The results demonstrate that the present strategy has great potential for constructing multiplex nucleic acid micro/nano biosensors for local and in situ detection of multiple nucleic acid molecules such as miRNAs at a time.