4 years ago

DEK protein level is a biomarker of CD138<sup>positive</sup> normal and malignant plasma cells

Emrah Özçelik, Ahmet Özdilek, Annette S. Kim, Amma Bosompem, Gerard Grosveld, Ayten Kandilci, Zihni Onur Çalışkaner, Türkan Çakar, Bülent Saka, Öner Doğan

by Zihni Onur Çalışkaner, Türkan Çakar, Emrah Özçelik, Ahmet Özdilek, Annette S. Kim, Öner Doğan, Amma Bosompem, Gerard Grosveld, Bülent Saka, Ayten Kandilci

Overexpression of DEK oncogene is associated with increased proliferation of carcinoma cells and it is observed in several solid tumors due to the amplification of the 6p22.3 chromosomal region where DEK locates. Although the same chromosomal amplification occurs in multiple myeloma (MM), a plasma cell neoplasm, whether the expression and the copy number of the DEK gene are affected in MM remains elusive. We show that despite the increased copy number in CD138positive MM cells (4 out of 41 MM samples), DEK mRNA expression was down-regulated compared with that in CD138negative bone marrow (BM) cells of the same patients (P<0.0001). DEK protein was not detectable by immunohistochemistry (IHC) in CD138positive normal plasma cells or in malignant plasma cells of MM patients (n = 56) whereas it was widely expressed in normal and neoplastic B-cells. Stable knockdown or overexpression of DEK in CD138positive MM cell lines did not affect the proliferation and viability of the cells profoundly in the presence or absence of chemotherapeutic agent melphalan whereas knockdown of DEK moderately but significantly increased the expression level of CD138 (p<0.01). Decreased DEK expression in plasma cells suggests a potential role of this gene in plasma cell development and lack of detectable DEK protein by IHC could be used as a biomarker for normal and malignant plasma cells.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.pone.0178025

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