3 years ago

An Oxidative Central Metabolism Enables Salmonella to Utilize Microbiota-Derived Succinate

An Oxidative Central Metabolism Enables Salmonella to Utilize Microbiota-Derived Succinate
Caroline C. Gillis, Maria G. Winter, Tatiane Furtado de Carvalho, Daniel P. Beiting, Helene L. Andrews-Polymenis, Daniela Chessa, Elizabeth R. Hughes, Sebastian E. Winter, Lora V. Hooper, Jiwoong Kim, Luisella Spiga, Cassie L. Behrendt, Renato L. Santos, Wenhan Zhu

Summary

The mucosal inflammatory response induced by Salmonella serovar Typhimurium creates a favorable niche for this gut pathogen. Conventional wisdom holds that S. Typhimurium undergoes an incomplete tricarboxylic acid (TCA) cycle in the anaerobic mammalian gut. One change during S. Typhimurium-induced inflammation is the production of oxidized compounds by infiltrating neutrophils. We show that inflammation-derived electron acceptors induce a complete, oxidative TCA cycle in S. Typhimurium, allowing the bacteria to compete with the microbiota for colonization. A complete TCA cycle facilitates utilization of the microbiota-derived fermentation product succinate as a carbon source. S. Typhimurium succinate utilization genes contribute to efficient colonization in conventionally raised mice, but provide no growth advantage in germ-free mice. Mono-association of gnotobiotic mice with Bacteroides, a major succinate producer, restores succinate utilization in S. Typhimurium. Thus, oxidative central metabolism enables S. Typhimurium to utilize a variety of carbon sources, including microbiota-derived succinate.

Publisher URL: http://www.cell.com/cell-host-microbe/fulltext/S1931-3128(17)30301-3

DOI: 10.1016/j.chom.2017.07.018

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.