4 years ago

UV-Induced RPA1 Acetylation Promotes Nucleotide Excision Repair

UV-Induced RPA1 Acetylation Promotes Nucleotide Excision Repair
Jiajia Wang, Hanqing He, Ting Liu


Replication protein A (RPA) is a multifunctional, single-stranded DNA-binding protein complex and plays a critical role in DNA replication and damage response. Herein, we show that the 70-kDa subunit of RPA (RPA1) is acetylated on lysine 163 by the acetyltransferases GCN5 and PCAF and that such acetylation is reversed principally via the action of the deacetylase HDAC6. UV irradiation promotes cytoplasmic translocation of HDAC6, thereby disrupting the interaction of HDAC6 with RPA1 and increasing RPA1 acetylation. Mutation of the acetylation site of RPA1 specifically impairs the ability of the protein to interact with the key nucleotide excision repair (NER) protein XPA, reduces XPA retention at sites of DNA damage caused by UV, compromises NER, and renders the cell hypersensitive to UV irradiation. Our data suggest that the acetylation status of RPA1 played a crucial role in repair of DNA damage via NER.

Publisher URL: http://www.cell.com/cell-reports/fulltext/S2211-1247(17)31108-7

DOI: 10.1016/j.celrep.2017.08.016

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