3 years ago

Casposons: mobile genetic elements that gave rise to the CRISPR-Cas adaptation machinery

Casposons: mobile genetic elements that gave rise to the CRISPR-Cas adaptation machinery
A casposon, a member of a distinct superfamily of archaeal and bacterial self-synthesizing transposons that employ a recombinase (casposase) homologous to the Cas1 endonuclease, appears to have given rise to the adaptation module of CRISPR-Cas systems as well as the CRISPR repeats themselves. Comparison of the mechanistic features of the reactions catalyzed by the casposase and the Cas1–Cas2 heterohexamer, the CRISPR integrase, reveals close similarity but also important differences that explain the requirement of Cas2 for integration of short DNA fragments, the CRISPR spacers.

Publisher URL: www.sciencedirect.com/science

DOI: S1369527416301710

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