5 years ago

Comparison of methods available for the identification of Mycobacterium chimaera

Mycobacterium chimaera is a recently described nontuberculous mycobacterium belonging to the M. avium complex (MAC). Because this species is implicated in a worldwide outbreak due to contaminated heater-cooler unit water tanks during open-heart surgery, it becomes mandatory for clinical microbiology laboratories to be able to differentiate M. chimaera from the other MAC species, especially M. intracellulare. Such identification was so far restricted to specialized laboratories, since it required the analysis of several gene sequences. The aim of this study was to evaluate commercialized methods for identifying M. chimaera with regard to the reference gene sequencing ITS, the internal transcribed spacer 16-23S. Methods 47 clinical and environmental isolates including 41 MAC were identified using (i) PCR sequencing of the ITS gene and of the hsp65 gene, (ii) three molecular biology kits (INNO-LiPA Mycobacteria, GenoType Mycobacterium CM and GenoType NTM-DR), and (iii) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using MicroFlex software. Results There was a high concordance for species determination between the reference ITS sequencing and the GenoType NTM-DR test (39/41, 95%), the INNO-LiPA test (38/41, 93%) and the hsp65 sequencing (38/41, 93%). The GenoType CM test did not distinguish M. chimaera from M. intracellulare. MALDI-TOF MS distinguished two M. chimaera - M. intracellulare groups separated from M. avium and from the other mycobacterial species on a score-oriented dendrogram, but did not differentiate the two species either. Conclusions This study showed that INNO-LiPA and Genotype NTM-DR are efficient assays for M. chimaera identification in clinical microbiology laboratories.

Publisher URL: www.sciencedirect.com/science

DOI: S1198743X17304172

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.